#Amplifx 100 for bass Pc

Grangemard I, Wallach J, Maget-Dana R, Peypoux F (2001) Lichenysin: a more efficient cation chelator than surfactin. Giacomodonato MN, Pettinari J, Souto GI, Meandez SB, Loapez NI (2001) A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere. Gancel F, Montastruc L, Liu T, Zhao L, Nikov I (2009) Lipopeptide overproduction by cell immobilization on iron-enriched light polymer particles. Proc Natl Acad Sci USA 96:13294–13299Įshita SM, Roberto NH, Beale JM, Mamiya BM, Workman RF (1995) Bacillomycin L c, a new antibiotic of the iturin group: Isolations, structures, and antifungal activities of the congeners. Arch Microbiol 191:63–71ĭuitman EH, Hamoen LW, Rembold M, Venema G, Seitz H, Saenger W, Bernhard F, Reinhardt R, Schmidt M, Ullrich C, Stein T, Leenders F, Vater J (1999) The mycosubtilin synthetase of Bacillus subtilis ATCC 6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. J Biotechnol 140:27–37Ĭhollet-Imbert M, Gancel F, Slomianny C, Jacques Ph (2009) Differentiated pellicle organization and lipopeptide production in standing culture of Bacillus subtilis strains. J Bacteriol 188:4024–4036Ĭhen XH, Koumoutsi A, Scholz R, Schneider K, Vater J, Süssmuth R, Piel J, Borriss R (2009) Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens. Nucl Acids Res 36:326–331Ĭhen XH, Vater J, Piel J, Franke P, Scholz R, Schneider K, Koumoutsi A, Hitzeroth G, Grammel N, Strittmatter AW, Gottschalk G, Süssmuth RD, Borriss R (2006) Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42. Comb Chem High Throughput Screen 6:541–556Ĭaboche S, Pupin M, Leclere V, Fontaine A, Jacques Ph, Kucherov G (2008) Norine: a database of non-ribosomal peptides. Activity–structure relationships to design new bioactive agents. J Ind Microbiol Biotechnol 34:443–56īonmatin JM, Laprevote O, Peypoux F (2003) Diversity among microbial cyclic lipopeptides: iturins and surfactins.

#Amplifx 100 for bass Pc

Microbial Ecol 49:10–24īarrios-Llerena ME, Burja AM, Wright PC (2007) Genetic analysis of polyketide synthase and peptide synthetase genes in cyanobacteria as a mining tool for secondary metabolites. In addition, this work highlighted the differences between the fengycin and plipastatin operon at DNA level.Īyuso-Sacido A, Genilloud O (2005) New PCR primers for screening of NRPS and PKS-I systems in actinomycetes: detection and distribution of these biosynthetic gene sequences in major taxonomic groups. It also led to the discovery of the presence of non-ribosomal peptide synthetase genes in Bacillus thuringiensis serovar berliner 1915 and in Bacillus cereus LMG 2098.

strains, this technique was used successfully to detect not only the expected genes in the lipopeptide producing strains but also the presence of a plispastatin gene in Bacillus subtilis ATCC 21332 and a gene showing a high similarity with the polyketide synthase type I gene in the B. The comparative bioinformatics analyses of each operon led to the design of four primer pairs for the three families taking into account the differences between open reading frames of each synthetase gene. To detect strains able to produce these lipopeptides, a new polymerase chain reaction screening approach was developed using degenerated primers based on the intraoperon alignment of adenylation and thiolation nucleic acid domains of all enzymes implicated in the biosynthesis of each lipopeptide family. These biosurfactants show a broad spectrum of biological activities. Bacillus strains produce non-ribosomal lipopeptides that can be grouped into three families: surfactins or lichenysins, iturins and fengycins or plispastatins.